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Coding

Part:BBa_J45013:Design

Designed by: Stephen Payne   Group: iGEM06_MIT   (2006-06-08)


PAL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2090
    Illegal BamHI site found at 352
    Illegal XhoI site found at 403
    Illegal XhoI site found at 466
    Illegal XhoI site found at 484
    Illegal XhoI site found at 562
    Illegal XhoI site found at 763
    Illegal XhoI site found at 1006
    Illegal XhoI site found at 1753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 922
    Illegal NgoMIV site found at 1168
    Illegal NgoMIV site found at 1336
    Illegal NgoMIV site found at 1479
    Illegal NgoMIV site found at 1813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 463
    Illegal BsaI site found at 793
    Illegal BsaI site found at 799
    Illegal BsaI.rc site found at 1924
    Illegal SapI site found at 753


Design Notes

The purpose of this part would be to develop cinnamic acid from natural food for E. coli. From there, if we can find the SAM:CCMT part, we could develop a cinnamon smell from E. coli merely from the cells eating their natural food.


Source

If we received such a part, it would probably be donated by Faulkner, et al. If not, the part would need to be modified to successfully express it in E. coli. GENBANK Accession Number = X12702

References

Orum H and Rasmussen OF. Expression in E. coli of the gene encoding phenylalanine ammonia-lyase from Rhodosporidium toruloides. Appl Microbiol Biotechnol 1992 Mar; 36(6) 745-7. pmid:1368015. PubMed HubMed [orum92]

Rasmussen OF and Oerum H. Analysis of the gene for phenylalanine ammonia-lyase from Rhodosporidium toruloides. DNA Seq 1991; 1(3) 207-11. pmid:1773059. PubMed HubMed [rasmussen91]

Faulkner JD, Anson JG, Tuite MF, and Minton NP. High-level expression of the phenylalanine ammonia lyase-encoding gene from Rhodosporidium toruloides in Saccharomyces cerevisiae and Escherichia coli using a bifunctional expression system. Gene 1994 May 27; 143(1) 13-20. pmid:8200528. PubMed HubMed [faulkner94]